Comparative evaluation of desensitizing Dente 91 tooth paste and Dente 91 mouthwash in the occlusion of dentinal tubules. In- vitro study

Abstract

Background: Dentine hypersensitivity is one of the most painful long-term problems of the teeth and has the 
lowest success rate.1 Dentine hypersensitivity (DH) is defined as a “short sharp pain arising from exposed dentine 
most commonly at the tooth cervical area in response to thermal, tactile evaporative, osmotic or chemical stimuli 
but which cannot be ascribed to any other dental defects, diseases or restorative treatments”.2 
Aim 
The aim of the present is to evaluate and compare the desensitizing Dente 91 tooth paste and Dente 91 mouthwash 
in the occlusion of dentinal tubules under scanning electron microscope (SEM). 
Materials and Methods 
Extracted premolars were collected and stored in 10% formalin. Dentin blocks measuring 8 mm × 5 mm× 2 mm 
were prepared from the crown part adjacent to CEJ. The specimens were randomly divided into 3 groups. Group1 -Dente 91 Toothpaste group, Group 2 – Dente 91 Mouthwash group and Group 3 - Combination group. The 
samples were treated twice daily as per the assigned group for 21 days. The percentage of the occluded dentinal 
tubules and the open area of tubules was assessed at baseline, 7th, 14th and 21st day by subjecting the samples for 
electro micrograph through scanning electron microscope. 
Results 
Combination group (Dente 91 tooth paste and Dente 91 mouthwash) showed higher percentage of blocked 
dentinal tubules and least area of open tubules as compared to toothpaste group alone followed by mouthwash 
group at 7th, 14th and 21st day respectively. 
Conclusion 
The study concluded that toothbrushing twice daily with dente 91 toothpaste along with usage of dente 91 
mouthwash for duration of 21 days effectively block the open dentinal tubule in order to decrease dentinal 
hypersensitivity.